Supporting Information Aptamer Degradation Inhibiting Combined with DNAzyme Cascade-Based Signal Amplification for Colorimetric Detection of Protein

نویسندگان

  • Jishan Li
  • Yuhua Jia
  • Jing Zheng
  • Wenwan Zhong
  • Guoli Shen
  • Ronghua Yang
  • Weihong Tan
چکیده

synthesized from Takara Biotechnology Co. Ltd. (Dalian, China). Recombinant human PDGF-BB and PDGF-AB were purchased from Bowling Vaccine & Pharmaceutical Inc. (San Francisco, CA). Bovine serum albumin (BSA), human serum albumin (HSA), human immunoglobulin G (IgG), thrombin, bovine serum were obtained from Dingguo Biotechnology Co. Ltd. (Beijing, China). Escherichia coli Exonuclease I (Exo I) was obtained from New England Biolabs (Ipswich, MA). SYBR Gold (supplied as 10,000×concentrate in DMSO) gel stain for polyacrylamide gel electrophoresis (PAGE) was obtained from Invitrogen (Carlsbad, CA). 2,2′-azinobis(3-ethylbenzothiozoline)-6-sulfonic acid (ABTS), Polyacrylic acid (PAA), diethylene glycol (DEG), and mercaptoacetic acid (MAA) were purchased from Sigma-Aldrich Chemical Co. H2O2, hemin, tris(hydroxymethyl) aminomethane (Tris), HEPES, Lead acetate (PbAc2), reagents for making the stopping solution and denatured gel, and all other chemicals were of analytical grade and obtained from Sinopharm Chemical Reagent Co. Ltd (Shanghai, China). All solutions were prepared using ultrapure water, which was obtained through a Millipore Milli-Q water purification system (electrical resistance of >18.3 MΩ) (Billerica, MA).

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تاریخ انتشار 2013